Composite

Part:BBa_K5427075:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)

pTac+ RBS1_12000 + Spider Silk + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1132
    Illegal NheI site found at 1155
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1943
    Illegal BsaI.rc site found at 1116
    Illegal SapI.rc site found at 1211


Design Notes

Not applicable.

Source

BBa_J23119

Ryu, M.-H., & Gomelsky, M. (2014). Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications. ACS Synthetic Biology, 3(11), 802–810. https://doi.org/10.1021/sb400182x

Arndt, T., Greco, G. H., Schmuck, B., Bunz, J., Shilkova, O., Francis, J., Pugno, N., Kristaps Jaudzems, Barth, A., Johansson, J., & Rising, A. (2022). Engineered Spider Silk Proteins for Biomimetic Spinning of Fibers with Toughness Equal to Dragline Silks. Advanced Functional Materials, 32(23), 2200986–2200986. https://doi.org/10.1002/adfm.202200986

BBa_J428326